Research

Fall 2021

Our group is interested in studying how the behaviors of individual cells contribute to the formation of tissue patterns. This is an essential process required for the development,  homeostasis, and regeneration of living tissues. We use the fruit fly, Drosophila Melanogaster, as a model organism alongside genetics, microscopy, and quantitative analyses to ask questions like:

  1. How do cells communicate with each other during patterning events?

  2. How does cell shape and movement affect cell-cell communication?

  3. Are there rules that individual cells follow to make fate decisions during patterning?

  4. How do these processes contribute to the self-organization of the tissue?

We currently have students investigating the role of Myosins in the cell shape changes that are essential for Notch-mediated lateral inhibition. We also are investigating the role of Notch interactors on pattern formation. We have plans to pursue collaborative experiments using mathematical modeling and tissue engineering strategies to investigate what factors help drive different patterns and how we can manipulate these factors to change the dynamics and output of patterning processes.

Microscope
 

Recent publications

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Phosphorylation and Proteolytic Cleavage of Notch in Canonical and Noncanonical Notch Signaling

The Notch signaling pathway is deceptively simple, but can contribute to a diverse array of biological functions. In this review, we broke down two key events in Notch signaling: proteolytic cleavage and phosphorylation.
Hunter and Giniger, 2020 Notch Signaling in Embryology and Cancer

March 2020

A role for actomyosin contractility in Notch signaling

January 2019

The mechanism of Notch activation requires a force producing step. We wondered whether the activity of non-muscle myosin II on the actin cytoskeleton, which can produce forces in cells, played a role in this process. Using the developing fly bristle pattern and tools in cell culture, we find that myosin II activity is important for normal Notch signaling and pattern formation.
Hunter et al, 2019 BMC Biology

Coordinated control of Notch-Delta signalling and cell division aids lateral inhibition mediated tissue patterning

What 'locks in' a pattern as it develops? Here we found that cells communicating to each other during bristle patterning are only allowed to signal to each other until they divide into two daughter cells. The Notch signaling that occurs is linked to the progression of the cell cycle. We used genetics and mathematical models to show that if you unlink these two processes, the pattern is disrupted.
Hunter et al, 2016 Development

May 2016

Ion channels contribute to the regulation of cell sheet forces during Drosophila dorsal closure

January 2014

How do noisy, individual cell behaviors coordinate to drive a smooth, robust, tissue movement? We identified several genes encoding channel subunits associated with mechanically gated ion channels that regulate cell shape and tissue tension generation during a morphogenetic process in fruit fly embryogenesis.
Hunter et al, 2014 Development